High-salt organic waste is a kind of pollutants that difficult to treat. In order to explore the feasibility of microbial transformation to treat high-salt organic waste, this study used the marine yeast H117as the research object, and Rhodotorula minuta was identified through morphological observation, physiological and biochemical analysis, and 26S rRNA sequence analysis. On this basis, the colony count analysis of R. minuta H117 was conducted in the simulated high-salt organic wastewater, high-salt soy sauce wastewater and high-salt soy sauce residue, and the results showed that R. minuta H117 could tolerate 9% (m/v) salt concentration, and the maximum viable cell number was 2.91×109cfu/mL in the high-salt soy sauce residue as the base material, while the number of colonies in the high salt soy sauce wastewater was lower relatively. This study provides data support for further research on the microecological preparations for mariculture using the Rhodotorula minuta H117 for transformation of high salt organic wastes. [Chinese Fishery Quality and Standards, 2024, 14(3): 01-07]