Fish scale labeling is one of the key techniques for the proliferation and release of aquatic organisms. In order to investigate the safety and effectiveness of the strontium otolith labeling technique during the proliferation and release of Myxocyprinus asiaticus, individuals with a mean body length of (10.47±1.91) cm and a mean body weight of (12.53±5.80) g were selected as the study subjects. The strontium concentration in the SrCl₂?6H₂O solution was progressively increased to five different gradients: 0, 12, 18, 36, and 72mg/L, respectively, to assess the impact of strontium labeling on Myxocyprinus asiaticus at different strontium concentrations. The results showed: (1) No significant differences were observed in the sucker region of Myxocyprinus asiaticus among the different strontium concentrations; (2) Quantitative line analysis revealed that the Sr/Ca ratio in the experimental group exposed to 12-72 mg/L strontium concentration exhibited a distinct peak value in specific areas. As the exogenous strontium concentration increased, the Sr/Ca ratio at the edge of the otolith first increased and then stabilized; (3) Following the change of time after labeling, the content of strontium in muscle showed a trend of initial increasing and then decreasing, with the strontium residue in the muscle of the experimental group, at strontium concentrations of 12-18 mg/L, being completely metabolized within 7 d. It is suggested that a strontium concentration of 12 mg/L and a duration of 7 d should be chosen as optimal conditions for labeling exogenous strontium. ［Chinese Fishery Quality and Standards, 2024, 14(2): 22-27］