丁香酚对四指马鲅幼鱼的麻醉效果及抗氧化影响
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1.农业农村部南海渔业资源开发利用重点实验室;2.大连海洋大学水产与生命学院

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国家重点研发计划“蓝色粮仓科技创新”重点专项(2018YFD0900200);广东省省级乡村振兴战略专项资金种业振兴项目(项目编号:2022-SPY-00-013);中国水产科学研究院基本科研业务费资助项目(2023TD58).通讯作者:区又君(1964-),女,研究员,从事鱼类生物学、发育生物学与水产增养殖技术研究. E-mail: ouyoujun@126.com第一作者;谢希尧(1997-),男,硕士研究生,研究方向为鱼类生物学. E-mail: xiyaoxie999@163.com


Effects of eugenol on anaesthesia and antioxidation of Eleutheronema tetradactylum
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    摘要:

    为研究丁香酚对四指马鲅幼鱼(Eleutheronema tetradactylum)的麻醉和镇静作用,以及丁香酚对幼鱼鳃组织损伤和肝脏抗氧化能力的影响,试验比较了在(30和35)℃条件下5个不同质量浓度的丁香酚(5、10、15、20、25? mg.L?1)对平均全长(6±0.324?cm )、平均体质量为(1.6±0.336?g)的四指马鲅幼鱼的静水麻醉效应,并根据幼鱼在丁香酚麻醉液中的行为表现,进一步细化丁香酚浓度。试验结果表明:在(30和35)℃条件下,1)随着丁香酚质量浓度的升高,幼鱼进入同一麻醉时期的时间显著降低(P<0.05);2)丁香酚对幼鱼的最适麻醉浓度不超过0.1?mg.L?1,且使用该浓度进行持续麻醉12?h后和复苏后24?h幼鱼存活率均为100%;3)不同浓度的丁香酚显著提高了幼鱼肝脏抗氧化酶(SOD、CAT、GPX和T-AOC)活性(P<0.05);4)观察幼鱼组织病理切片发现:随着丁香酚浓度的增加,鳃小片会出现弯曲、末端肿大、严重时会涨破和脱落等现象,使用丁香酚浓度为0.1?mg.L?1对幼鱼进行静水模拟运输第6?h时鳃小片会出现轻微的弯曲、水肿和排列紧密现象,但在模拟运输第12?h后这些情况都会得到改善;

    Abstract:

    To investigate the anesthetic and sedative effects of eugenol on juvenile Eleutheronema tetradactylum, as well as its effects on gill tissue damage and liver antioxidant capacity in juvenile fish, five different concentrations of Eugenol (5,10,15,20,25mg.L?1) were used to anesthetize juvenile fish with an average body weight of (1.6 ± 0.336 g) and an average total length of (6 ± 0.324 cm) at (30.35) ° C, the concentration of Eugenol was further refined according to its behavior in Eugenol. The experimental results show that: under the conditions of (30 and 35) ° C, 1) with the increase of Eugenol concentration, the time of juvenile entering the same period of anesthesia was significantly decreased (P<0.05); 2) the optimal anesthetic concentration of Eugenol for juvenile was 0.1mg.L?1, 3) the activities of liver antioxidant enzymes (SOD, CAT, GPX and T-AOC) were significantly increased in different concentrations of Eugenol (P<0.05 ); 4) with the increase of Eugenol concentration, gills were damaged. When Eugenol concentration was 14mg.L?1, the gills of juvenile fish were slightly damaged at the 6 h, but the damage was improved after the 12 h.

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  • 收稿日期:2024-01-15
  • 最后修改日期:2024-04-16
  • 录用日期:2024-05-13
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