The stress and immune parameters in juvenile Seriola dumerili at different concentrations of eugenol (0, 0.012 5, 0.025 0, 0.037 5 and 0.050 0 μL/mL) were investigated in the simulated transportation experiment . The results showed that the survival rate of the juvenile S. dumerili was 100% in all groups after 8h transportation. The concentrations of CRH, ATCH and COR in the serum of juvenile S. dumerili were higher than those in the D25 and D50 group (P＜0.05). The expression of heme oxygenase (HO-1), glucocorticoid (GR) and heat shock protein (HSP70, HSP90) genes in the liver and gills of juvenile S. dumerili were significantly different (P＜0.05), while the expression level of haptoglobin (HP) decreased together with other indicators to maintain the dynamic balance in juvenile amberjack. The expression of lysozyme (Lyz), hepcidin (Hep), nuclear factor κB1 (NF-Kb1), tumor necrosis factor (TNF-α) and interleukin-1β (IL-1β) genes in S. dumerili generally increased first and then decreased with the increase of eugenol concentration. The increased expression levels of complement 3 (C3), transforming growth factor β1 (TGF-β1), interleukin 10 (IL-10) reflected that the body produced a large number of harmful substances due to stress during transportation. The expression levels of major histocompatibility complex 1 (MHC-1) and interferon-γ (IFN-γ) first decreased and then increased with the increase of eugenol concentration. In conclusion, the effects of eugenol on stress and immunity in the transport of juvenile S. dumerili were mainly manifested by the inhibition of oxidative stress and inflammation, thus causing changes in immune-related genes. The stress response of juvenile S. dumerili was the least and the immune status was the best in the D50 group. Therefore, eugenol is suitable as an anesthetic for transporting juvenile S. dumerili, and the concentration of eugenol should be 0.025 0 μL/mL in the sealed bag airtight oxygenated transport mode.[Chinese Fishery Quality and Standards, 2023, 13(3)：18-25]