Sera from 80 fish-allergic and only shrimp and crab-allergic patients were collected in Zhejiang region, and the binding activities of IgE in patients sera to sarcoplasmic proteins from 7 species of fish were examined by enzyme-linked immunosorbent assay (ELISA). The protein composition and allergen distribution of fish were analyzed by SDS-PAGE and Western blot. Results demonstrated that there was no significant difference in specific IgE positive rate between fish allergic and only shrimp and crab-allergic patients. But allergic patients’ sera had different positive rate in reaction with different kinds of fish proteins. The proteins of Oncorhynchus mykiss, Scophthalmus maximus and Micropterus salmoides were more allergenic with the positive rate of 70.67%, 46.67% and 26.39%, respectively. In constrast, Aristichthys nobilis, Cyprinus carpio, Ctenopharyngodon idellus and Acipenser sinensis had relatively low positive rate. SDS-PAGE analysis showed that the composition of protein was different in 7 fish species. Western blot results showed that the components of fish species had various reaction with positive sera. Thereinto, three species of fish (Oncorhynchus mykiss, Scophthalmus maximus and Micropterus salmoides), which was higher positive rate, were detected about 12 kDa specific protein bands. In summary, the study was detected and analyzed the specific IgE binding capacity in sera from fish-allergic and shrimp and crab-allergic patients to sarcoplasmic protein of varieties of fish. These provided some references for the diseases prevention of aquatic products and the development of hypoallergenic aquatic food.