A reversed-phase high performance liquid chromatographic (HPLC) method was established to determine tryptamine, 2-phenylethylamine, putrescine, cadaverive, histamine, tyramine, spermidine and spermine in salted-dried fish and the extraction method of biogenic amines was optimized. Biogenic amines in salted-dried fish were derived with dansyl chloride. 1,7-Diaminoheptane was used as an internal standard(IS). 8 biogenic amines were separated by C18 column(250 mm×4.6 mm, 5 μm), and then detected by fluorescence detector with excitation wavelength of 350 nm and emission wavelength of 520 nm. Gradient elute using a mobile phase composed of acetonitrile, ammonium acetate and ultrapure water was at 1 mL/min flow rate. Sodium hydroxide and saturated sodium bicarbonate were used to adjust pH of derivative system and biogenic amines were extracted with trichloroacetic acid (TCA) or perchloric acid (HClO4) or hydrochloric acid (HCl). The results indicated that 8 biogenic amines completely separated within 30 min, and the correlation coefficient (R2) was greater than 0.998 66; linear range of spermine was among 0.5~50.0 μg/mL; linear range of the other biogenic amines were among 0.5~100.0 μg/mL. The limits of detection were in the range of 0.03~0.10 μg/mL. The relative standard deviation (RSD) of precision was lower than 1.87%. The pH 11.0 was most suitable for derivative system. The using of 5% TCA for extracting biogenic amines in salted-dried fish was a more suitable extraction than the others. Biogenic amines in seven salted-dried fish were determined effectively and the values of total biogenic amines were in the range of (91.75±3.11)~(986.43±5.42) mg/kg. So this optimized method is a rapid, efficient detection way for 8 biogenic amines in salted-dried aquatic product. ［Chinese Fishery Quality and Standards, 2014, 4(4):41-48］