Abstract: In vitro whole-bionic digestion model was used to study the bioaccessibility of fluorine and its health risk assessment in Euphausia superba. Fluorine content was determined by ion selection election. The results showed that a great difference existed in different extracts for the dissolved fluorine content and its bioavailability. The dissolved fluorine content in the gastric extracts was between 81.68 and 1 987.4 mg/kg, and its bioaccessibility was between 61.24% and 87.50%. The dissolved fluorine content in the intestinal extracts was between 23.48 and 550.64 mg/kg, and its bioaccessibility was between 16.97% and 27.31%. The fluorine content in the gastric extracts was related to the shrimp shell. Considering the fluorine content and bioavailability in the gastric extracts, the contribution rate of fluorine in E. superba to the children tolerable daily intake (TDI) was below 0.035%, and if considering the fluorine content and bioavailability in the intestinal extracts, the contribution rate of fluorine in E. superba to the children TDI was below 0.30%. So, the human health risk of fluorine in E. superba with different processing method as in the present experiment was low. If the daily intake content of the shrimp without shell doesn’t exceed 8 000 mg, it is safe for an average person. The present result provides important information for the safety evaluation of fluoride in E. superba, and the deep processing and the utilization of E. superba. [Chinese Fishery Quality and Standards, 2014, 4(4):21-26]