Abstract: The study aimed to establish a flow injection analysis ( FIA) detection method of chromium (Ⅵ) in water samples. Chromium (Ⅵ) can cause a color reaction with the diphenyl carbazide（DPC）—which has the maximum absorption wavelength at 540 nm and there was a linear relationship between the degree of color and chromium (Ⅵ) concentration. The time used for a single sample analysis was 133s and frequency of determination was 27 samples / h. The range of measurement was from 0μg/L to 400.0μg/L and the regression equation was y=0.1255x-0.00401，with a correlation coefficient of 0.999 98. The detection limits of the method was 0.4 μg/L.The relative standard deviation ( RSD) was 0.59% and the recovery of standard addition in water sample was 91.0% —108 %. This FIA detection method of chromium (Ⅵ) was a simple，accurate and highly selective method, especially suitable for laboratory detection of a large number of samples, with practicality. [Chinese Fishery Quality and Standards, 2012, 2(4):]