Abstract:In this study Seriola dumerili juveniles, (10.34±1.33) cm in length and (19.00±3.00) g in weight were transported in polyethylene bags with airtight oxygenation, to investigate the effects of three different transport time of 4 h, 8 h and 12 h on stress, metabolism, antioxidants and immunity. The results showed that ammonia nitrogen (NH4-N) concentrations in the water increased with increasing transport time, while pH and dissolved oxygen (DO) decreased. In liver tissues, the expression levels of glucocorticoid receptor (GR) and pyruvate kinase (PK) were significantly higher in the 4 h group than in the 8 h and 12 h groups (P ＜0.05); the expression levels of copper superoxide dismutase (Cu-SOD) and manganese superoxide dismutase (Mn-SOD) were significantly higher in the 12 h group than in the 4 h and 8 h groups (P ＜0.05); Malondialdehyde (MDA) concentration was significantly higher in the 12 h group than in the 4 h and 8 h groups (P＜0.05); major histocompatibility complex (MHC-1) was significantly different between the 4 h, 8 h and 12 h groups (P ＜0.05). In the gills, the expression levels of GR and PK were significantly higher in the 8 h group than in the 4 h and 12 h groups; the expression levels of Cu-SOD were significantly higher in the 8 h group than in the 4 h and 12 h groups (P ＜0.05); the expression levels of Mn-SOD were significantly lower in the 4 h group than in the 8 h and 12 h groups (P ＜0.05); the MDA concentration was significantly higher in the 12 h group than in the 4 h and 8 h groups; the expression level of MHC-1 in the 12 h group was significantly lower than that in the 4 h and 8 h groups. As the time increases, the fish will affect the water quality (pH, ammonia and nitrogen, dissolved oxygen) during transportation, leading to damage to the liver and gills of the fish. Therefore, it is best to control the transportation within 8 h to reduce the damage to the physiological function and immune function of fish.[Chinese Fishery Quality and Standards, 2023, 13(2):25-36]