Abstract:In this study, the same batch of artificially bred Eleutheronema tetradactylum were reared in ponds with a salinity of 7.0 and 12.0 respectively, and the amino acid and fatty acid compositions of E.tetradactylum were compared,to explore the nutritional composition in the muscle of Eleutheronema tetradactylum cultured in two sets of salinity pond, and the results can be used in the culture of E.tetradactylum pond. The same batch of juvenile E. tetradactylum were cultured in the low salinity water (7, 12), and the amino acid and fatty acid compositions of E.tetradactylum were analyzed.In this study,we found three phenomena, the first is there were no significant differences in the body composition, amino acid and fatty acid content of E.tetradactylum between the two groups (P>0.05),the second is 16 amino acids were detected in the muscle of E.tetradactylum. The contents of glutamate (Glu), aspartic acid (ASP), lysine (Lys) and leucine (Leu) were rich in the samples, accounting for 16.2%, 11.1%, 10.1% and 8.7% of the total amino acids, respectively. Moreover, the contents of essential amino acid (EAA),Non-essential amino acids (NEAA) and delicious amino acids(DAA) were 8.07 g/100g, 11.74 g/100g and 7.80 g/100g, respectively.The EAA/TAA and EAA/NEAA of the two sets of salinity in the muscle of E.tetradactylum respectively are 0.407 and 0.797,the last is, contents of unsaturated fatty acids (UFA) and polyunsaturated fatty acids (PUFA) in the muscle were 1.90 g/100g and 1.13 g/100g, respectively. DHA and EPA accounted for 5.63% and 2.86% of the total fatty acids, respectively.The results indicate that small difference in salinity will not significantly affect the nutritional compostion in muscle of E.tetradactylum. However, the nutritive value of E.tetradactylum. cultured in brackish pond was slightly higher, indicating that E.tetradactylum cultured in low salinity has more nutritional and promotion value.[Chinese Fishery Quality and Standards,2023,13(2):11-17]