As a kind of safe and efficient green feed additive, enzyme preparation has been widely used in livestock and poultry feed. Enzyme preparation can not only alleviate the shortage of feed resources in China to a certain extent, but also promote the development of green breeding environment. Due to the restriction of heat sensitivity, the application rate of enzyme preparation in aquatic feed is low. However, with the application of some equipment (e.g., multichannel vacuum post spraying), the wide application of liquid enzyme preparation in aquatic feed has become possible. This paper reviews the application, mechanism of action and existing problems of enzyme preparation in aquatic animals, and makes a prospect for its application in aquatic feed, in order to provide reference for the application of feed enzyme preparation. [Chinese Fishery Quality and Standards, 2021, 11(1):61-67]