This paper introduced the application of highly specific indirect enzymelinked immunosorbent assay for the simultaneous detection of four nitrofuran metabolites, chloramphenicol and florfenicol, which established a method to quickly analyze the residues of six substances in aquatic products. Briefly, 1 g of samples was digested with hydrochloric acid, then extracted with ethyl acetate, and detected using an enzymelinked immunosorbent kit. The experimental results showed that the residual amount of the six compounds were all in good linear range with more than 0.995. Specifically, the recovery range of chloramphenicol (CAP) in tissue samples at the concentratioins of 0.1, 0.5, and 1.5 μg/kg was 60.00% to 126.26%. The recovery range of florfenicol (FF) in the tissue samples at the three concentrations of 0.5, 2.0, and 8.0 μg/kg was 60.83% to 68.37%. Tissue samples were spiked with furancillin (SEM) at 0.1, 0.5, and 2.0 μg/kg. The recoveries were 65.66% to 148.16%. Tissue samples were spiked with furanodone (AMOZ) at 0.2, 1.0, and 4.0 μg/kg, and the recovery range was 69.18% to 140.50%. Tissue samples were spiked with furanotoin (AHD) at the concentrations of 0.2, 1.0, and 4.0 μg/kg with the recovery range from 62.50% to 131.00%. Tissue samples with furazolidone (AOZ) at the three concentrations of 0.1, 0.5, and 2.0 μg/kg showed the recoveries ranged from 83.1% to 144.0%. The relative standard deviations of the above six veterinary drug residues were between 0 and 7.5%. This method is applicable to the quick determination of the metabolites including nitrofuran, chloramphenicol, and florfenicol in aquatic products. [Chinese Fishery Quality and Standards,2021,11(1):27-33]