In order to evaluate the protective effect of resveratrol on lipopolysaccharide (LPS)induced inflammation in the kidney cells of grass carp (Ctenopharyngodon idella) (CIK cells), we established the model of LPSinduced inflammation in CIK cells, and assessed the influence of resveratrol addition in the CIK culture medium by the antioxidant parameters and inflammatoryrelated factors mRNA expression. Results showed that the LPS treatment resulted in a decrease of the CIK cells viability (P<0.05), and an increase of the activity of LDH compared with control groups (P<0.05). Moreover, the intracellular activity of CAT and SOD and the intracellular concentration of GSH were decreased (P<0.05), while the content of MDA was increased (P<0.05). The transcriptional data showed that LPS treatment could significantly upregulate the expression of CAT, TNFα, IFNγ and IL1 genes (P<0.05), but there was not significant effect on the transcriptional expression of IL10 and SOD genes in CIK cells (P>0.05). When resveratrol (4 μg/mL) was added in the culture medium, it could significantly attenuate the damage of CIK cells induced by LPS, maintain the activity of antioxidant enzymes (SOD and CAT) and the amount of antioxidants (GSH), hold down the expression of TNFα and other inflammationrelated genes to the normal level (P<0.05), and promote the expression of CAT gene (P<0.05). This study showed that resveratrol had a significant intervention effect on CIK cell damage induced by LPS, which mainly maybe by the means of the improvement of antioxidant capacity of CIK cells and inhibition of the expression of proinflammation factors in CIK cells. The study could provide a theoretical basis for the application of resveratrol in the prevention and treatment of fish diseases characterized by inflammation and oxidative damage. [Chinese Fishery Quality and Standards,2021,11(1):18-26]