China，as a    major aquaculture    country，has  huge   demand      for     feed in       aquaculture，but    tiiere      still       exists    cer­tain hidden   danger    of chemical    mildew   preventive.     Now there    are   rich  edible seaweed     resources.   The  study was in­tended to develop novel edible seaweed mildew preventive and prove its inhibition on toxin - producing Fusarium spp. By adopting the antimicrobial substance culture met!iod，and measuring its bacterial colony arc lengt!i and growt!i inhi­bition ratio，we    investigated   the   relationship between  inhibitory  concentration    and arc lengt!i  of     inhibition   zone   when medium was   added   to the         edible seaweed    mildew   preventive.   The   inhibition  effect of kelp   content，iodine content  and pH was  measured by Box - Behnken  test.Results showed    the      negative    correlation between   inhibitory  concentration and arc length of inhibition zone and the mildew preventive with kelp and iodine ( K +1) was more effective than otii-ers. And kelp content was more effective in inhibition. Then the inhibition ratio on the five diferent Fusarium % IBE2,   IBE8,   JX-6JX189-6-landJX-9)   were 6385% ,  70.00% , 5880% , 60. 00%and69. 44% ,whichIBE2 was the most active, that IBE2 was inhibited remarkably  than   other "-0+-.，and     its MIC   was 137.67  mg/mL.

The study provided reference for a safer and more effective edible seaweed mildew preventive for the various fields in agriculture, thus solvin/ the F—sarium spp. poiution of feedin/ Litopenae—svannamei effectively. It will contribute to the aquaculture of Litopenae—svannamei. ' Chinese Fishery Quality and Standards, 2015 , 5 % 3 ):54 - 62 ]