采用高效液相色谱荧光检测器（HPLC-FLD）法测定鲈组织中残留的阿维菌素，研究其在鲈体内的富集和消除规律。使用0.5 μg/L阿维菌素对初始体重为（120±15）g的鲈连续药浴20 d，停药后，采用高效液相色谱法测定肌肉、肝脏、鳃和血液组织中阿维菌素的残留量。结果表明，阿维菌素能够在鲈肌肉、肝脏、血液和鳃各组织中富集，但不同组织对阿维菌素的富集能力不同，肝脏的蓄积能力最强，而肌肉的蓄积能力最弱。肝脏中阿维菌素最高质量浓度（Cmax）可达87.36 μg/kg，药时曲线下面积（AUC肝脏）为12 977.73 (μg/L)·h，而肌肉Cmax仅为7.74 μg/kg，AUC肌肉仅为2 105.75 (μg/L)·h，肌肉、鳃、肝脏和血液对阿维菌素的消除半衰期分别为6.9、9.5、8.2和10.7 d。由于影响水生生物药代动力学的因素较多，同种药物在不同水生生物体内的药代动力学参数有很大差异。鲈在15~18 ℃条件下连续药浴阿维菌素20 d，可食组织肌肉中阿维菌素的残留量40 d后降至检测限以下。本研究为今后在水产养殖中合理使用阿维菌素药物提供了理论依据
The accumulation and elimination rules of avermectin in perch (Lateolabraxjaponicus) were studied and the avermectin residues in perch were determined with HPLC-fluorescence method. The perch with initial body weight of (120±15) g were dealt with 0.5 μg/L medicated bath for 20 d. After medicated bath was terminated, the avermectin retained in muscle, liver, gill and blood was determined. The results showed that avermectin could accumulate in muscle, liver, gill and blood of perch, but the enrichment capacity of each tissue was different. The enrichment capacity of avermectin was comparatively strong in the liver, but relatively weak in muscle. The Cmax of liver could reach 87.36 μg/kg，and AUCliver was 12 977.73 (μg/L)·h, whereas the Cmax of muscle was 7.74 μg/kg and AUCmuscle was only 2 105.75 (μg/L)·h. Elimination half-time of avermectin in the muscle, gill, liver and blood of perch were 6.9, 9.5, 8.2 and 10.7 d, respectively. Since many factors affected residues of aquatic animals, pharmacokinetic parameters of the same drug in different aquatic animals were very different. In this condition, when the temperature were 15-18℃, the residues of avermectin in muscle dropped to detection limit after 40 d. This study discussed the pharmacokinetics of avermectin in perch, and analyzed the content variations of the muscle, liver, gill and blood in perch with different time. The dynamic change rules of avermectin were clarified, which would provide theoretical basis for clinical medication in future.
邢丽红,孙伟红,冷凯良*,等.阿维菌素在鲈组织中的富集及消除规律研究[J].中国渔业质量与标准,2013,3(4):63-69. XING Lihong, SUN Weihong, LENG Kailiang*,et al. Accumulation and elimination rules of avermectin in perch (Lateolabraxjaponicus)[J]. Chinese Fishery Quality and Standards,2013,3(4):63-69.复制