Abstract: A high performance liquid chromatography (HPLC) method for the determination of Mequindox (MEQ) residues in aquatic products was developed. The samples were extracted by mixed solution of acetonitrile-ethyl acetate. Clean-up was carried out with n-hexane and the analyte was determined by HPLC. The linear relationship between the peak area and concentration was good in the range of 50µg/L －1000µg/L. The limit of quantitation was 20µg/kg. The mean recovery rate was over 70.0% within the range of 20－100µg/kg, and the relative standard deviation rate was less than 10%. The method is simple, fast, sensitive, with good repeatability and reliable results, thus can meet the requirement of mass and fast analysis in the laboratory.